Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma-Driven Biocatalysis
Abdulkadir Yayci (Ruhr-Universität Bochum)
Tim Dirks (Ruhr-Universität Bochum)
Friederike Kogelheide (Ruhr-Universität Bochum)
Miguel Alcalde (Campus de Cantoblanco)
F Hollmann (TU Delft - BT/Biocatalysis)
Peter Awakowicz (Ruhr-Universität Bochum)
Julia E. Bandow (Ruhr-Universität Bochum)
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Abstract
The use of a microscale atmospheric pressure plasma jet (μAPPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the μAPPJ offered significantly higher H2O2 production rates and better handling of larger reaction volumes. The performance of the μAPPJ was evaluated with recombinant unspecific peroxygenase from Agrocybe aegerita (rAaeUPO). Using plasma-treated buffer, no side reactions with other plasma-generated species were detected. For long-term treatment, rAaeUPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the μAPPJ. The enzyme had a turnover of 36,415 mol mol−1 and retained almost full activity even after prolonged plasma treatment. Overall, the μAPPJ presents a promising plasma source for plasma-driven biocatalysis.
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