Protease and phenol production of Cynara cardunculus L. Cell suspension in a chemostat

Journal article (1996)

Authors

M. E. Lima Costa University of the Algarve
W.M. van Gulik OLD BT/Cell Systems Engineering
M. S.S. Pais University of Lisbon
J. M.S. Cabral Instituto Superior Técnico (IST)
Research Group
OLD BT/Cell Systems Engineering
Chemostat Cynara cardunculus Phenols Plant cell suspension Proteases
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Published Date

1996

Language

English

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Research Group

OLD BT/Cell Systems Engineering

Abstract

Proteases from Cynara cardunculus L. are used in cheese-making. The growth of C. cardunculus cell suspension culture and the production of proteases and phenols were studied in sucrose-limited chemostat cultures at two dilution rates. Biomass yields and biomass and protease productivities in chemostat cultures were compared with those in batch cultures. C. cardunculus cells showed a high ability to adapt to chemostat conditions. Phenolic production was not observed in the steady-state cultures of C. cardunculus cell cultures in contrast to batch cultures. Protease production was linked to the exponential growth phase and was higher and more stable in sucrose-limited chemostats than in batch culture.