3D visualization of plant-pathogen interaction inside plant leaves with dynamic contrast optical coherence tomography
J. de Wit (TU Delft - ImPhys/Kalkman group, TU Delft - ImPhys/Computational Imaging)
Sebastian Tonn (Universiteit Utrecht)
Mon-Ray Shao (Universiteit Utrecht)
Guido van den Ackerveken (Universiteit Utrecht)
J. Kalkman (TU Delft - ImPhys/Computational Imaging, TU Delft - ImPhys/Kalkman group)
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Abstract
Optical coherence tomography (OCT) can image deep inside scattering plant tissue with micrometer resolution. However, conventional OCT lacks specificity to distinguish plant tissue from pathogen tissue. Here we show how dynamic OCT (dOCT) creates functional contrast of Bremia, a downy mildew in lettuce, based on sub-resolution dynamic activity inside the tissue. We demonstrate its applicability for disease resistance quantification and longitudinal study of pathogen growth.