Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens
M. Quintavalla (Istituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle Ricerche)
P. Pozzi (TU Delft - Team Raf Van de Plas)
M Verhaegen (TU Delft - Team Raf Van de Plas)
H.K. Bijlsma (TU Delft - Team Raf Van de Plas)
Hans Verstraete (TU Delft - Team Raf Van de Plas)
S. Bonora (Istituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle Ricerche)
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Abstract
Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL) that can be inserted right after the objective and works in conjunction with an image optimization software allowing for a wavefront sensorless correction. We presented the results obtained on several commercial microscopes among which a confocal microscope, a fluorescence microscope, a light sheet microscope and a multiphoton microscope.