Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens

Conference paper (2018)

Authors

M. Quintavalla CNR-Institute of Photonics and Nanotechnology
P. Pozzi Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
M.H.G. Verhaegen Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
H.K. Bijlsma Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
H.R.G.W. Verstraete Team Raf Van de Plas - Mechanical, Maritime and Materials Engineering
S. Bonora CNR-Institute of Photonics and Nanotechnology
Research Group
Team Raf Van de Plas (Mechanical, Maritime and Materials Engineering) (TU Delft)
Copyright: © 2018 M. Quintavalla, P. Pozzi, M.H.G. Verhaegen, H.K. Bijlsma, H.R.G.W. Verstraete, S. Bonora
DOI: https://doi.org/10.1117/12.2290036
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Published Date

2018

Language

English

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Faculty

Mechanical, Maritime and Materials Engineering

Department

Delft Center for Systems and Control

Research Group

Team Raf Van de Plas

Abstract

Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL) that can be inserted right after the objective and works in conjunction with an image optimization software allowing for a wavefront sensorless correction. We presented the results obtained on several commercial microscopes among which a confocal microscope, a fluorescence microscope, a light sheet microscope and a multiphoton microscope.

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