Biocatalytically induced surface modification of the tobacco mosaic virus and the bacteriophage M13

Journal Article (2019)
Research Group
BT/Biocatalysis
Copyright
© 2019 Valentina Vignali, Barbara S. Miranda, Irene Lodoso-Torrecilla, Cathelijn A.J. Van Nisselroy, Bas Jan Hoogenberg, Sybren Dantuma, F. Hollmann, Jan Willem De Vries
DOI related publication
https://doi.org/10.1039/c8cc08042a
More Info
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Publication Year
2019
Language
English
Copyright
© 2019 Valentina Vignali, Barbara S. Miranda, Irene Lodoso-Torrecilla, Cathelijn A.J. Van Nisselroy, Bas Jan Hoogenberg, Sybren Dantuma, F. Hollmann, Jan Willem De Vries
Research Group
BT/Biocatalysis
Issue number
1
Volume number
55
Pages (from-to)
51-54
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Abstract

Engineered viruses are finding an increasing number of applications in basic, translational research and materials science. Genetic and chemical engineering of capsids represents a key point for tailoring the properties of viral particles, but the synthetic efforts and limits accompanying these processes still hinder their usability. Here, a single-step highly selective biocatalytic functionalization approach is described, providing a general platform for virus-acrylate hybrid particles. The tobacco mosaic virus (TMV) and the bacteriophage M13 have been successfully modified via laccase induced free radical formation on the tyrosine residues through single electron oxidation as the initiating step and the free radicals subsequently react with acrylate-based monomers. This new approach can be extended to other biomolecular assemblies with surface exposed tyrosine residues, when the introduction of new functionalities is desired.

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