Automated sub-5 nm image registration in integrated correlative fluorescence and electron microscopy using cathodoluminescence pointers

Journal Article (2017)
Author(s)

M.T. Haring (TU Delft - ImPhys/Charged Particle Optics)

N Liv (TU Delft - ImPhys/Charged Particle Optics)

A.C. Zonnevylle (TU Delft - ImPhys/Charged Particle Optics)

A.C. Narvaez Gonzalez (TU Delft - ImPhys/Charged Particle Optics)

LM Voortman (Delmic)

P Kruit (TU Delft - ImPhys/Charged Particle Optics)

J. P. Hoogenboom (TU Delft - ImPhys/Charged Particle Optics)

Research Group
ImPhys/Charged Particle Optics
Copyright
© 2017 M.T. Haring, N. Liv Hamarat, A.C. Zonnevylle, A.C. Narvaez Gonzalez, L.M. Voortman, P. Kruit, J.P. Hoogenboom
DOI related publication
https://doi.org/10.1038/srep43621
More Info
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Publication Year
2017
Language
English
Copyright
© 2017 M.T. Haring, N. Liv Hamarat, A.C. Zonnevylle, A.C. Narvaez Gonzalez, L.M. Voortman, P. Kruit, J.P. Hoogenboom
Research Group
ImPhys/Charged Particle Optics
Volume number
7
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Abstract

In the biological sciences, data from fluorescence and electron microscopy is correlated to allow fluorescence biomolecule identification within the cellular ultrastructure and/or ultrastructural analysis following live-cell imaging. High-accuracy (sub-100 nm) image overlay requires the addition of fiducial markers, which makes overlay accuracy dependent on the number of fiducials present in the region of interest. Here, we report an automated method for light-electron image overlay at high accuracy, i.e. below 5 nm. Our method relies on direct visualization of the electron beam position in the fluorescence detection channel using cathodoluminescence pointers. We show that image overlay using cathodoluminescence pointers corrects for image distortions, is independent of user interpretation, and does not require fiducials, allowing image correlation with molecular precision anywhere on a sample.