A possible influence of extracellular polysaccharides on the analysis of intracellular metabolites from Trichoderma harzianum grown under carbon-limited conditions
Lucas Gelain (TU Delft - OLD BT/Cell Systems Engineering, University of Campinas)
José Geraldo da Cruz Pradella (Universidade Federal de Sao Paulo)
Aline Carvalho da Costa (University of Campinas)
Luuk van der Wielen (TU Delft - BT/Bioprocess Engineering, University of Limerick)
Walter M. van Gulik (TU Delft - BT/Industriele Microbiologie)
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Abstract
Intracellular metabolites were evaluated during the continuous growth of Trichoderma harzianum P49P11 under carbon-limited conditions. Four different conditions in duplicate were investigated (10 and 20 g/L of glucose, 5.26/5.26 g/L of fructose/glucose and 10 g/L of sucrose in the feed). Differences in the values of some specific concentrations of intracellular metabolites were observed at steady-state for the duplicates. The presence of extracellular polysaccharide was confirmed in the supernatant of all conditions based on FT-IR and proton NMR. Fragments of polysaccharides from the cell wall could be released due to the shear stress and since the cells can consume them under carbon-limited conditions, this could create an unpredictable carbon flow rate into the cells. According to the values of the metabolite concentrations, it was considered that the consumption of those fragments was interfering with the analysis.
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