Assessing the influence of visual stimulus properties on steady-state visually evoked potentials and pupil diameter

Abstract

Steady-State Visual Evoked Potentials (SSVEPs) are brain responses measurable via electroencephalography (EEG) in response to continuous visual stimulation at a constant frequency. SSVEPs have been instrumental in advancing our understanding of human vision and attention, as well as in the development of brain-computer interfaces (BCIs). Ongoing questions remain about which type of visual stimulus causes the most potent SSVEP response. The current study investigated the effects of color, size, and flicker frequency on the signal-to-noise ratio of SSVEPs, complemented by pupillary light reflex measurements obtained through an eye-tracker. Six participants were presented with visual stimuli that differed in terms of color (white, red, green), shape (circles, squares, triangles), size (10,000 to 30,000 pixels), flicker frequency (8 to 25 Hz), and grouping (one stimulus at a time versus four stimuli presented in a 2 × 2 matrix to simulate a BCI). The results indicated that larger stimuli elicited stronger SSVEP responses and more pronounced pupil constriction. Additionally, the results revealed an interaction between stimulus color and flicker frequency, with red being more effective at lower frequencies and white at higher frequencies. Future SSVEP research could focus on the recommended waveform, interactions between SSVEP and power grid frequency, a wider range of flicker frequencies, a larger sample of participants, and a systematic comparison of the information transfer obtained through SSVEPs, pupil diameter, and eye movements.