Pupil mask diversity for image correction in microscopy
Dean Wilding (TU Delft - Mechanical Engineering)
Paolo Pozzi (TU Delft - Mechanical Engineering)
Oleg Soloviev (ITMO University, TU Delft - Mechanical Engineering, Flexible Optical B.V.)
Gleb Vdovin (ITMO University, TU Delft - Mechanical Engineering, Flexible Optical B.V.)
Michel Verhaegen (TU Delft - Mechanical Engineering)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
Three-dimensional microscopy suffers from sample-induced aberrations that reduce the resolution and lead to misinterpretations of the object distribution. In this paper, the resolution of a three-dimensional fluorescent microscope is significantly improved by introducing an amplitude diversity in the form of a binary amplitude mask positioned in several di erent orientations within the pupil, followed by computer processing of the diversity images. The method has proved to be fast, easy to implement, and cost-e ective in high-resolution imaging of casper fli:GFP zebrafish.
help_outline