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Pupil mask diversity for image correction in microscopy

Journal Article (2018)
Author(s)

D. Wilding (TU Delft - Team Raf Van de Plas)

P. Pozzi (TU Delft - Team Raf Van de Plas)

Oleg Soloviev (ITMO University, TU Delft - Team Raf Van de Plas, Flexible Optical B.V.)

Gleb Vdovin (ITMO University, TU Delft - Team Raf Van de Plas, Flexible Optical B.V.)

M.H.G. Verhaegen (TU Delft - Team Raf Van de Plas)

Research Group
Team Raf Van de Plas
Copyright
© 2018 D. Wilding, P. Pozzi, O.A. Soloviev, Gleb Vdovin, M.H.G. Verhaegen
DOI related publication
https://doi.org/10.1364/OE.26.014832
More Info
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Publication Year
2018
Language
English
Copyright
© 2018 D. Wilding, P. Pozzi, O.A. Soloviev, Gleb Vdovin, M.H.G. Verhaegen
Research Group
Team Raf Van de Plas
Issue number
12
Volume number
26
Pages (from-to)
14832-14841
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Abstract

Three-dimensional microscopy suffers from sample-induced aberrations that reduce the resolution and lead to misinterpretations of the object distribution. In this paper, the resolution of a three-dimensional fluorescent microscope is significantly improved by introducing an amplitude diversity in the form of a binary amplitude mask positioned in several di erent orientations within the pupil, followed by computer processing of the diversity images. The method has proved to be fast, easy to implement, and cost-e ective in high-resolution imaging of casper fli:GFP zebrafish.