Efficient Aerobic Oxidation of trans-2-Hexen-1-ol using the Aryl Alcohol Oxidase from Pleurotus eryngii
T. Almeida (TU Delft - BT/Biocatalysis)
M.M.C.H. Van Schie (TU Delft - BT/Biocatalysis)
A. Ma (External organisation, Student TU Delft)
Florian Tieves (TU Delft - BT/Biocatalysis)
Sabry H.H. Younes (Sohag University, TU Delft - BT/Biocatalysis)
Elena Fernandez Fernandez Fueyo (TU Delft - BN/Greg Bokinsky Lab, TU Delft - BT/Biocatalysis)
I. W.C.E. Arends (Universiteit Utrecht)
A. Riul (University of Campinas)
F Hollmann (TU Delft - BT/Biocatalysis)
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Abstract
The selective oxidation of trans-2-hexen-1-ol to the corresponding aldehyde using a recombinant aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) is reported. Especially using the two liquid phase system to overcome solubility and product inhibition issues enabled to achieve more than 2.200.000 catalytic turnovers for the production enzyme as well as molar product concentrations, pointing towards an economic feasible reaction.