Bienzymatic Cascade Combining a Peroxygenase with an Oxidase for the Synthesis of Aromatic Aldehydes from Benzyl Alcohols

Journal article (2023)

Authors

Yunjian Ma Macau University of Science and Technology, South China University of Technology
Zongquan Li South China University of Technology
Hao Zhang South China University of Technology
Vincent Kam Wai Wong Macau University of Science and Technology
F. Hollmann BT/Biocatalysis - AS
Yonghua Wang South China University of Technology, Guangdong Youmei Institute of Intelligent Bio-manufacturing Co, Foshan
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2023 Yunjian Ma, Zongquan Li, Hao Zhang, Vincent Kam Wai Wong, F. Hollmann, Yonghua Wang
DOI
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https://doi.org/10.3390/catal13010145
AaeUPO Aromatic aldehydes Cascade catalysis Flavour compounds PeAAOx
More Info
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Published Date

2023

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

Aromatic aldehydes are important aromatic compounds for the flavour and fragrance industry. In this study, a parallel cascade combining aryl alcohol oxidase from Pleurotus eryngii (PeAAOx) and unspecific peroxygenase from the basidiomycete Agrocybe aegerita (AaeUPO) to convert aromatic primary alcohols into high-value aromatic aldehydes is proposed. Key influencing factors in the process of enzyme cascade catalysis, such as enzyme dosage, pH and temperature, were investigated. The universality of PeAAOx coupled with AaeUPO cascade catalysis for the synthesis of aromatic aldehyde flavour compounds from aromatic primary alcohols was evaluated. In a partially optimised system (comprising 30 μM PeAAOx, 2 μM AaeUPO at pH 7 and 40 °C) up to 84% conversion of 50 mM veratryl alcohol into veratryl aldehyde was achieved in a self-sufficient aerobic reaction. Promising turnover numbers of 2800 and 21,000 for PeAAOx and AaeUPO, respectively, point towards practical applicability.