Polarized light imaging and two-photon fluorescence microscopy correlative approach for 3D reconstruction of the orientation of myelinated fibers

Conference paper (2017)

Authors

Irene Costantini University of Florence

Miriam Menzel Forschungszentrum Jülich

Ludovico Silvestri University of Florence

Nicole Schubert Forschungszentrum Jülich

Markus Axer Forschungszentrum Jülich

Katrin Amunts Heinrich Heine University, Forschungszentrum Jülich

Francesco S. Pavone University of Florence, CNR-INO

Affiliation

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Published Date

2017

Language

English

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Abstract

In this work, we employ an integrated label-free dual approach that combines 3DPolarized light imaging with two-photon fluorescence microscopy to study the mixture of various fiber orientations within the sample of interest.