Polarized light imaging and two-photon fluorescence microscopy correlative approach for 3D reconstruction of the orientation of myelinated fibers

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Polarized light imaging and two-photon fluorescence microscopy correlative approach for 3D reconstruction of the orientation of myelinated fibers

Conference Paper (2017)

Author(s)

Irene Costantini (University of Florence)

Miriam Menzel (Forschungszentrum Jülich)

Ludovico Silvestri (University of Florence)

Nicole Schubert (Forschungszentrum Jülich)

Markus Axer (Forschungszentrum Jülich)

Katrin Amunts (Heinrich Heine University, Forschungszentrum Jülich)

Francesco S. Pavone (Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche, University of Florence)

DOI related publication

https://doi.org/10.1364/BRAIN.2017.BrW4B.5 Final published version

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https://resolver.tudelft.nl/uuid:be7efa6b-032f-47ba-9a9c-818c67f4097a

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Publication Year

2017

Language

English

ISBN (electronic)

9781557528209

Event

Downloads counter

173

Abstract

In this work, we employ an integrated label-free dual approach that combines 3DPolarized light imaging with two-photon fluorescence microscopy to study the mixture of various fiber orientations within the sample of interest.