Towards a synthetic cell cycle
Lorenzo Olivi (Wageningen University & Research)
Mareike Berger (AMOLF Institute for Atomic and Molecular Physics)
Ramon N.P. Creyghton (Systems Biophysics, AMOLF Institute for Atomic and Molecular Physics)
N. De Franceschi (TU Delft - BN/Cees Dekker Lab, Kavli institute of nanoscience Delft)
C. Dekker (TU Delft - BN/Cees Dekker Lab, Kavli institute of nanoscience Delft)
Bela M. Mulder (AMOLF Institute for Atomic and Molecular Physics)
Nico J. Claassens (Wageningen University & Research)
Pieter Rein ten Wolde (AMOLF Institute for Atomic and Molecular Physics)
John van der Oost (Wageningen University & Research)
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Abstract
Recent developments in synthetic biology may bring the bottom-up generation of a synthetic cell within reach. A key feature of a living synthetic cell is a functional cell cycle, in which DNA replication and segregation as well as cell growth and division are well integrated. Here, we describe different approaches to recreate these processes in a synthetic cell, based on natural systems and/or synthetic alternatives. Although some individual machineries have recently been established, their integration and control in a synthetic cell cycle remain to be addressed. In this Perspective, we discuss potential paths towards an integrated synthetic cell cycle.
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