Hybrid Pore formation by directed insertion of a-haemolysin into solid-state nanopores

Journal article (2010)

Authors

AR Hall BN/Cees Dekker Lab - AS
A Scott External organisation
D Rotem External organisation
K Mehta External organisation
H Bayley External organisation
C. Dekker BN/Cees Dekker Lab - AS
Research Group
BN/Cees Dekker Lab (AS) (TU Delft)
More Info
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Published Date

2010

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Cees Dekker Lab

Abstract

Most experiments on nanopores have concentrated on the pore-forming protein ¿-haemolysin (¿HL)1 and on artificial pores in solid-state membranes2. While biological pores offer an atomically precise structure3 and the potential for genetic engineering4, solid-state nanopores offer durability, size and shape control5, and are also better suited for integration into wafer-scale devices. However, each system has significant limitations: ¿HL is difficult to integrate because it relies on delicate lipid bilayers for mechanical support, and the fabrication of solid-state nanopores with precise dimensions remains challenging. Here we show that these limitations may be overcome by inserting a single ¿HL pore into a solid-state nanopore. A double-stranded DNA attached to the protein pore is threaded into a solid-state nanopore by electrophoretic translocation. Protein insertion is observed in 30¿40% of our attempts, and translocation of single-stranded DNA demonstrates that the hybrid nanopore remains functional. The hybrid structure offers a platform to create wafer-scale device arrays for genomic analysis, including sequencing6.