Adsorption of free floating DNA and antimicrobial resistance genes out of wastewater effluents by sewage-based biochar and iron-oxide-coated sands

Master Thesis (2019)
Author(s)

A. Seeram Apoorva (TU Delft - Civil Engineering & Geosciences)

Contributor(s)

David G. Weissbrodt – Mentor (TU Delft - BT/Environmental Biotechnology)

David Calderon Franco – Mentor (TU Delft - BT/Environmental Biotechnology)

Gertjan Medema – Graduation committee member (TU Delft - Sanitary Engineering)

R.E.F. Lindeboom – Graduation committee member (TU Delft - Sanitary Engineering)

Faculty
Civil Engineering & Geosciences
Copyright
© 2019 Apoorva Seeram Apoorva
More Info
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Publication Year
2019
Language
English
Copyright
© 2019 Apoorva Seeram Apoorva
Graduation Date
24-10-2019
Awarding Institution
Delft University of Technology
Programme
['Civil Engineering | Environmental Engineering']
Faculty
Civil Engineering & Geosciences
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Abstract

The presence of extracellular DNA (eDNA) containing antibiotic resistance genes in the treated wastewater effluents can contribute to the spread of antimicrobial resistance (AMR) among receiving waters. The removal of cell associated antibiotic resistance genes (ARGs) has been widely studied using advance treatments. However, these treatments were not evaluated for cell free or extracellular ARGs resulting from the cell lysis or secretion during metabolic activities. eDNA is known to well adsorb onto clay, suspended particles and other soil components. Thus, in this research the potential and the main mechanisms involved in the removal of eDNA by adsorption onto sewage-based biochar and iron-oxide-coated sands has been studied.

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