Adsorption of free floating DNA and antimicrobial resistance genes out of wastewater effluents by sewage-based biochar and iron-oxide-coated sands
undefined Seeram Apoorva (TU Delft - Civil Engineering & Geosciences)
David Weissbrodt – Mentor (TU Delft - Applied Sciences)
David Calderon Franco – Mentor (TU Delft - Applied Sciences)
Gertjan Medema – Graduation committee member (TU Delft - Civil Engineering & Geosciences)
Ralph Lindeboom – Graduation committee member (TU Delft - Civil Engineering & Geosciences)
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Abstract
The presence of extracellular DNA (eDNA) containing antibiotic resistance genes in the treated wastewater effluents can contribute to the spread of antimicrobial resistance (AMR) among receiving waters. The removal of cell associated antibiotic resistance genes (ARGs) has been widely studied using advance treatments. However, these treatments were not evaluated for cell free or extracellular ARGs resulting from the cell lysis or secretion during metabolic activities. eDNA is known to well adsorb onto clay, suspended particles and other soil components. Thus, in this research the potential and the main mechanisms involved in the removal of eDNA by adsorption onto sewage-based biochar and iron-oxide-coated sands has been studied.