Telomeres stall DNA loop extrusion by condensin
Brian T. Analikwu (TU Delft - Applied Sciences, Kavli institute of nanoscience Delft)
Alice Deshayes (Université Paris Cité, Institut François Jacob, CEA, Inserm, Université Paris-Saclay, Paris)
Jaco van der Torre (TU Delft - Applied Sciences, Kavli institute of nanoscience Delft)
Thomas M. Guérin (Université Paris Cité, Inserm, Université Paris-Saclay, Paris, CEA, Institut François Jacob)
Allard J. Katan (Kavli institute of nanoscience Delft, TU Delft - Applied Sciences)
Roman Barth (Kavli institute of nanoscience Delft, TU Delft - Applied Sciences)
Stefano Mattarocci (Université Paris-Saclay, Paris, Inserm, Institut François Jacob, CEA, Université Paris Cité)
Cees Dekker (TU Delft - Applied Sciences, Kavli institute of nanoscience Delft)
Stéphane Marcand (CEA, Université Paris Cité, Inserm, Institut François Jacob, Université Paris-Saclay, Paris)
undefined More Authors
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
DNA loop extrusion by SMC proteins is a key process underlying chromosomal organization. It is unknown how loop extruders interact with telomeres where DNA is densely covered with proteins. Using complementary in vivo and in vitro single-molecule approaches, we study how loop-extruding condensin interacts with Rap1, the telomeric DNA-binding protein of Saccharomyces cerevisiae. We show that dense linear Rap1 arrays can completely halt DNA loop extrusion, with a blocking efficiency depending on the array length and the DNA gap size between proteins. In anaphase cells, dense Rap1 arrays are found to accumulate condensin and to cause a local chromatin decompaction, as monitored with a microscopy-based approach, with direct implications for the resolution of dicentric chromosomes produced by telomere fusions. Our findings show that linear arrays of DNA-bound proteins can efficiently halt DNA loop extrusion by SMC proteins, which may impact cellular processes from telomere functions to transcription and DNA repair.
help_outline