Plug-and-play adaptive optics for commercial laser scanning fluorescence microscopes based on an adaptive lens

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Plug-and-play adaptive optics for commercial laser scanning fluorescence microscopes based on an adaptive lens

Journal Article (2020)

Author(s)

P. Pozzi (Università Degli Studi di Modena e Reggio Emilia, TU Delft - Mechanical Engineering)

M. Quintavalla (IMAMOTER - C.N.R. Sensors and Nanomaterials Laboratory)

A. B. Wong (Erasmus MC)

J. G.G. Borst (Erasmus MC)

S. Bonora (IMAMOTER - C.N.R. Sensors and Nanomaterials Laboratory)

M. Verhaegen (TU Delft - Mechanical Engineering)

Research Group

Team Raf Van de Plas

DOI related publication

https://doi.org/10.1364/OL.396998 Final published version

To reference this document use

https://resolver.tudelft.nl/uuid:d1068735-0931-4752-82d6-4173f0967bb5

More Info

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Publication Year

2020

Language

English

Research Group

Team Raf Van de Plas

Issue number

13

Volume number

45

Pages (from-to)

3585-3588

Downloads counter

169

Abstract

In this Letter, we present a solution for simple implementation of adaptive optics in any existing laser scanning fluorescence microscope. Adaptive optics are implemented by the introduction of a multiactuator adaptive lens between the microscope body and the objective lens. Correction is performed with a sensorless method by optimizing the quality of the images presented on screen by the microscope software. We present the results acquired on both a commercial linear excitation confocal microscope and a custom-made multiphoton excitation microscope.