Distinct mobility patterns of BRCA2 molecules at DNA damage sites
Maarten W. Paul (Erasmus MC)
Jesse Aaron (Howard Hughes Medical Institute)
Eric Wait (Elephas Biosciences, Howard Hughes Medical Institute)
Romano M. van Genderen (Erasmus MC)
Arti Tyagi (Erasmus MC, TU Delft - BN/Kristin Grussmayer Lab, Kavli institute of nanoscience Delft)
Hélène Kabbech (Erasmus MC)
Ihor Smal (Erasmus MC)
Teng-Leong Chew (Howard Hughes Medical Institute)
Roland Kanaar (Erasmus MC)
Claire Wyman (Erasmus MC)
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Abstract
BRCA2 is an essential tumor suppressor protein involved in promoting faithful repair of DNA lesions. The activity of BRCA2 needs to be tuned precisely to be active when and where it is needed. Here, we quantified the spatio-temporal dynamics of BRCA2 in living cells using aberration-corrected multifocal microscopy (acMFM). Using multicolor imaging to identify DNA damage sites, we were able to quantify its dynamic motion patterns in the nucleus and at DNA damage sites. While a large fraction of BRCA2 molecules localized near DNA damage sites appear immobile, an additional fraction of molecules exhibits subdiffusive motion, providing a potential mechanism to retain an increased number of molecules at DNA lesions. Super-resolution microscopy revealed inhomogeneous localization of BRCA2 relative to other DNA repair factors at sites of DNA damage. This suggests the presence of multiple nanoscale compartments in the chromatin surrounding the DNA lesion, which could play an important role in the contribution of BRCA2 to the regulation of the repair process.
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