Fluorescence microscopy
A statistics-optics perspective
Mohamadreza Fazel (Arizona State University)
Kristin S. Grusmayer (TU Delft - BN/Bionanoscience, TU Delft - BN/Kristin Grussmayer Lab)
Boris Ferdman (Technion Israel Institute of Technology)
Aleksandra Radenovic (École Polytechnique Fédérale de Lausanne)
Yoav Shechtman (Technion Israel Institute of Technology)
Jörg Enderlein (Georg-August-University)
Steve Pressé (Arizona State University)
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Abstract
Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Accounting for these features is often critical in quantitatively interpreting microscopy images, especially those gathering information at scales on par with or smaller than light's emission wavelength. Here the optics responsible for generating fluorescent images, fluorophore properties, and microscopy modalities leveraging properties of both light and fluorophores, in addition to the necessarily probabilistic modeling tools imposed by the stochastic nature of light and measurement, are reviewed.