Biosynthesis of cyclic ketones by a H<sub>2</sub>O<sub>2</sub> self-sufficient cascade reaction

Journal article (2023)

Authors

Bin Wu South China University of Technology
Xiangyun Wang South China University of Technology
Bo Yang South China University of Technology
Yunjian Ma South China University of Technology
F. Hollmann BT/Biocatalysis - AS
Yonghua Wang Guangdong Youmei Institute of Intelligent Bio-manufacturing Co. Ltd., South China University of Technology
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2023 Bin Wu, Xiangyun Wang, Bo Yang, Yunjian Ma, F. Hollmann, Yonghua Wang
DOI
help_outline
https://doi.org/10.1016/j.mcat.2023.113396
Alcohol oxidase Unspecific peroxygenase Cyclohexanone HO self-cycling
More Info
expand_more

Published Date

2023

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

In the present work we propose a bienzymatic cascade for the oxyfunctionalisation of cycloalkanes to cyclic alcohols/cyclic ketones. By combining a H2O2-dependent peroxygenase with a O2-consuming and H2O2-producing alcohol oxidase an overall aerobic oxidation system was established. A convincing proof-of-concept is presented and some current limitations are outlined.