Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations

Review (2021)

Authors

Markus Hobisch Aarhus University
Dirk Holtmann University of Applied Sciences Mittelhessen, Giessen
Patricia Gómez de Santos Institute of Catalysis, CSIC, Madrid
Miguel Alcalde Institute of Catalysis, CSIC, Madrid
F. Hollmann BT/Biocatalysis - AS
Selin Kara Aarhus University
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2021 Markus Hobisch, Dirk Holtmann, Patricia Gomez de Santos, Miguel Alcalde, F. Hollmann, Selin Kara
DOI: https://doi.org/10.1016/j.biotechadv.2020.107615
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Published Date

2021

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

Peroxygenases are an emerging new class of enzymes allowing selective oxyfunctionalisation reactions in a cofactor-independent way different from well-known P450 monooxygenases. Herein, we focused on recent developments from organic synthesis, molecular biotechnology and reaction engineering viewpoints that are devoted to bring these enzymes in industrial applications. This covers natural diversity from different sources, protein engineering strategies for expression, substrate scope, activity and selectivity, stabilisation of enzymes via immobilisation, and the use of peroxygenases in low water media. We believe that peroxygenases have much to offer for selective oxyfunctionalisations and we have much to study to explore the full potential of these versatile biocatalysts in organic synthesis.