Resolution in super-resolution microscopy — definition, trade-offs and perspectives

Journal article (2024)

Authors

Kirti Prakash The Institute of Cancer Research and The Royal Marsden NHS Foundation Trust, Royal Marsden NHS Foundation Trust
David Baddeley The University of Auckland
Christian Eggeling Leibniz Institute for Photonic Technology (IPHT) Jena, Friedrich Schiller University Jena
Reto Fiolka University of Texas Southwestern
Rainer Heintzmann Leibniz Institute for Photonic Technology (IPHT) Jena, Friedrich Schiller University Jena
Suliana Manley EPFL Switzerland
Aleksandra Radenovic EPFL Switzerland
C.S. Smith Team Carlas Smith - Mechanical, Maritime and Materials Engineering
Hari Shroff Howard Hughes Medical Institute
Lothar Schermelleh University of Oxford
Research Group
Team Carlas Smith (Mechanical, Maritime and Materials Engineering) (TU Delft)
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Published Date

2024

Language

English

Reuse Rights

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Faculty

Mechanical, Maritime and Materials Engineering

Department

Delft Center for Systems and Control

Research Group

Team Carlas Smith

Abstract

Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM.