Resolution in super-resolution microscopy — definition, trade-offs and perspectives
K. Prakash (The Royal Marsden NHS Foundation Trust, The Institute of Cancer Research )
David Baddeley (The University of Auckland)
Christian Eggeling (Leibniz Institute for Photonic Technology (IPHT) Jena, Friedrich Schiller University Jena)
Reto Fiolka (University of Texas Southwestern)
Rainer Heintzmann (Leibniz Institute for Photonic Technology (IPHT) Jena, Friedrich Schiller University Jena)
Suliana Manley (École Polytechnique Fédérale de Lausanne)
Aleksandra Radenovic (École Polytechnique Fédérale de Lausanne)
C.S. Smith (TU Delft - Team Carlas Smith)
Hari Shroff (Howard Hughes Medical Institute)
Lothar Schermelleh (University of Oxford)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
Super-resolution microscopy (SRM) is gaining popularity in biosciences; however, claims about optical resolution are contested and often misleading. In this Viewpoint, experts share their views on resolution and common trade-offs, such as labelling and post-processing, aiming to clarify them for biologists and facilitate deeper understanding and best use of SRM.