MALDI TIMS IMS of Disialoganglioside Isomers GD1a and GD1b in Murine Brain Tissue

Journal Article (2023)
Authors

Katerina V. Djambazova (VanderBilt University)

Martin Dufresne (VanderBilt University)

L.G. Migas (TU Delft - Team Raf Van de Plas)

Angela R.S. Kruse (VanderBilt University)

Raf Van de Plas (TU Delft - Team Raf Van de Plas)

Richard M. Caprioli (VanderBilt University)

Jeffrey M. Spraggins (VanderBilt University)

Research Group
Team Raf Van de Plas
Copyright
© 2023 Katerina V. Djambazova, Martin Dufresne, L.G. Migas, Angela R.S. Kruse, Raf Van de Plas, Richard M. Caprioli, Jeffrey M. Spraggins
To reference this document use:
https://doi.org/10.1021/acs.analchem.2c03939
More Info
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Publication Year
2023
Language
English
Copyright
© 2023 Katerina V. Djambazova, Martin Dufresne, L.G. Migas, Angela R.S. Kruse, Raf Van de Plas, Richard M. Caprioli, Jeffrey M. Spraggins
Research Group
Team Raf Van de Plas
Issue number
2
Volume number
95
Pages (from-to)
1176-1183
DOI:
https://doi.org/10.1021/acs.analchem.2c03939
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Abstract

Gangliosides are acidic glycosphingolipids, containing ceramide moieties and oligosaccharide chains with one or more sialic acid residue(s) and are highly diverse isomeric structures with distinct biological roles. Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) enables the untargeted spatial analysis of gangliosides, among other biomolecules, directly from tissue sections. Integrating trapped ion mobility spectrometry with MALDI IMS allows for the analysis of isomeric lipid structures in situ. Here, we demonstrate the gas-phase separation and identification of disialoganglioside isomers GD1a and GD1b that differ in the position of a sialic acid residue, in multiple samples, including a standard mixture of both isomers, a biological extract, and directly from thin tissue sections. The unique spatial distributions of GD1a/b (d36:1) and GD1a/b (d38:1) isomers were determined in rat hippocampus and spinal cord tissue sections, demonstrating the ability to structurally characterize and spatially map gangliosides based on both the carbohydrate chain and ceramide moieties.

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