Use of digital micromirror devices as dynamic pinhole arrays for adaptive confocal fluorescence microscopy
Paolo Pozzi (TU Delft - Mechanical Engineering)
Dean Wilding (TU Delft - Mechanical Engineering)
Oleg Soloviev (Flexible Optical B.V., TU Delft - Mechanical Engineering, ITMO University)
Gleb Vdovin (TU Delft - Mechanical Engineering, Flexible Optical B.V., ITMO University)
Michel Verhaegen (TU Delft - Mechanical Engineering)
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Abstract
In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and scanning. A binary DMD is positioned in an image plane of the detection optical path, acting as a dynamic array of reflective confocal pinholes, images by a high performance CMOS camera. A second camera detects images of the light rejected by the pinholes for sensorless aberration correction.