E-MALDI

Optimized conditions during electrowetting-enhanced drop drying for MALDI-MS

Journal article (2017)

Authors

Olena Kudina University of Twente
H.B. Eral Intensified Reaction and Separation Systems - Mechanical, Maritime and Materials Engineering , Universiteit Utrecht
Frieder Mugele University of Twente
Research Group
Intensified Reaction and Separation Systems (Mechanical, Maritime and Materials Engineering) (TU Delft)
Copyright: © 2017 Olena Kudina, H.B. Eral, Frieder Mugele
DOI
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https://doi.org/10.1002/jms.3934
EMALDI Electrowetting-enhanced drop drying MALDI MALDI signal intensity increase ‘coffee stain’ effect suppression
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Published Date

2017

Language

English

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Faculty

Mechanical, Maritime and Materials Engineering

Department

Process and Energy

Research Group

Intensified Reaction and Separation Systems

Abstract

We recently showed that electrowetting-enhanced sample preparation for MALDI-MS (eMALDI) can increase the intensity of the MALDI signal by 2-25 times compared to conventional drop drying by concentrating all the dried sample in a single spot rather than leaving behind a heterogeneous coffee stain pattern. Here, we demonstrate that the eMALDI signal enhancement can be further increased to more than 100x by systematically optimizing the electrowetting actuation frequency and amplitude. This enables 30x signal increase for a peptide standard. Simultaneously drop drying times can be reduced approximately five times by increasing the actuation voltage and/or decreasing the initial drop volume.