Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications
Kirti Prakash (TU Delft - Team Carlas Smith)
David Baddeley (The University of Auckland)
Christian Eggeling (Friedrich Schiller University Jena, Leibniz Institute for Photonic Technology (IPHT) Jena)
Reto Fiolka (University of Texas Southwestern)
Rainer Heintzmann (Leibniz Institute for Photonic Technology (IPHT) Jena, Friedrich Schiller University Jena)
Suliana Manley (École Polytechnique Fédérale de Lausanne)
Aleksandra Radenovic (École Polytechnique Fédérale de Lausanne)
Hari Shroff (Howard Hughes Medical Institute)
Carlas Smith (TU Delft - Team Carlas Smith)
Lothar Schermelleh (University of Oxford)
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Abstract
Super-resolution microscopy (SRM) has undeniable potential for scientific discovery, yet still presents many challenges that hinder its widespread adoption, including technical trade-offs between resolution, speed and photodamage, as well as limitations in imaging live samples and larger, more complex biological structures. Furthermore, SRM often requires specialized expertise and complex instrumentation, which can deter biologists from fully embracing the technology. In this Perspective, a follow-up to our recent Q&A article, we aim to demystify these challenges by addressing common questions and misconceptions surrounding SRM. Experts offer practical insights into how biologists can maximize the benefits of SRM while navigating issues such as photobleaching, image artifacts and the limitations of existing techniques. We also highlight recent developments in SRM that continue to push the boundaries of resolution. Our goal is to equip researchers with the crucial knowledge they need to harness the full potential of SRM.
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