Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium

Journal article (2016)

Authors

Diana B. Muñiz-Márquez University Autonomous of Coahuila

Juan C. Contreras University Autonomous of Coahuila

Raúl Rodríguez University Autonomous of Coahuila

Solange Mussatto BT/Bioprocess Engineering -

José Teixeira University of Minho

Cristóbal N. Aguilar University Autonomous of Coahuila

Research Group

BT/Bioprocess Engineering () (TU Delft)

Aguamiel Fructooligosaccharides Fructosyltransferase Solid State Fermentation

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Published Date

2016

Language

English

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Bioprocess Engineering

Abstract

The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347U/L) was obtained at 32°C, using packing density of 0.7g/cm3. Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.