Non-marking Collection of Amino Acids from Fingerprints Using Hydrogels
W. Van Helmond (Nederlands Forensisch Instituut (NFI), Hogeschool van Amsterdam)
R. de Jong (Nederlands Forensisch Instituut (NFI))
JH Van Esch (TU Delft - ChemE/Advanced Soft Matter)
S. Oldenhof (TU Delft - ChemE/Advanced Soft Matter, Nederlands Forensisch Instituut (NFI))
Marcel De Puit (Nederlands Forensisch Instituut (NFI), TU Delft - OLD ChemE/Organic Materials and Interfaces)
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Abstract
The amino acid profile obtained from a fingerprint may provide valuable information on its donor. Unfortunately, the collection of chemicals from the fingerprint is often destructive to the fingerprint ridge detail. Herein we detail the use of cross-linkable solutions of dextran-methacrylate to form hydrogels capable of collecting amino acids from surfaces followed by extraction and quantification with UPLC-MS. This method allows for the amino acid profile analysis of fingerprints while allowing for their increased visualization at a later stage using the standard method of cyanoacrylate fuming followed by basic-yellow dyeing.
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