Optogenetics and Light-Sheet Microscopy
L. Maddalena (TU Delft - ImPhys/Carroll group)
Paolo Pozzi (Università Degli Studi di Modena e Reggio Emilia)
Nicolò G. Ceffa (External organisation)
Bas van der Hoeven (Student TU Delft)
E.C.M. Carroll (TU Delft - ImPhys/Carroll group)
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Abstract
Light-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in combination with light-sheet microscopy and discuss some current bottlenecks and horizons of light sheet in all-optical physiology. We describe how 3-dimensional optogenetics can be added to an home-built light-sheet microscope, including technical notes about choices in microscope configuration to consider depending on the time and length scales of interest.
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