H2as a fuel for flavin- And H2O2-dependent biocatalytic reactions

Journal Article (2020)
Author(s)

Ammar Al-Shameri (Technical University of Berlin)

Sebastien Willot (TU Delft - BT/Biocatalysis)

Caroline Paul (TU Delft - BT/Biocatalysis)

Frank Hollmann (TU Delft - BT/Biocatalysis)

Lars Lauterbach (Technical University of Berlin)

Research Group
BT/Biocatalysis
Copyright
© 2020 Ammar Al-Shameri, S.J. Willot, C.E. Paul, F. Hollmann, Lars Lauterbach
DOI related publication
https://doi.org/10.1039/d0cc03229h
More Info
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Publication Year
2020
Language
English
Copyright
© 2020 Ammar Al-Shameri, S.J. Willot, C.E. Paul, F. Hollmann, Lars Lauterbach
Research Group
BT/Biocatalysis
Issue number
67
Volume number
56
Pages (from-to)
9667-9670
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Abstract

The soluble hydrogenase from Ralstonia eutropha provides an atom efficient regeneration system for reduced flavin cofactors using H2 as an electron source. We demonstrated this system for highly selective ene-reductase-catalyzed CC-double bond reductions and monooxygenase-catalyzed epoxidation. Reactions were expanded to aerobic conditions to supply H2O2 for peroxygenase-catalyzed hydroxylations.