Application of Euler-Lagrange CFD for quantitative evaluating the effect of shear force on C. tinctorius L. cell in a stirred tank bioreactor

Journal article (2016)

Authors

Yu Liu East China University of Science and Technology
Ze-Jian Wang East China University of Science and Technology
J. Xia East China University of Science and Technology
C. Haringa ChemE/Transport Phenomena - AS
Ya-ping Liu Dalian University of Technology
Y. Chu East China University of Science and Technology
Y Zhuang East China University of Science and Technology
Si-Liang Zhang East China University of Science and Technology
Research Group
ChemE/Transport Phenomena (AS) (TU Delft)
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Published Date

2016

Language

English

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Faculty

Applied Sciences

Department

ChemE/Chemical Engineering

Research Group

ChemE/Transport Phenomena

Abstract

The relationship between shear environment and physiological characteristics of plant cells is always a crucial and challenging aspect in the scale up of suspension cultivations. In this work, we propose a quantitative method for evaluating the lethal effects of hydrodynamic on Carthamus tinctorius L. cell with computational fluid dynamic (CFD) technology and online capacitance viable cell detection. The study towards the effect of short term shear force on C. tinctorius L. cells was carried out in 5 L bioreactor under various overall shear intensities (0.02–1.82 w/kg), in which an online capacitance electrode was applied to count the dynamic vital cell. A first order kinetic model was achieved to describe the death rate in a time span of 60 min based on the capacitance. A product of the maximum shear stress and shear frequency (SSF) parameter, which is an evolution of energy dissipation/circulation function, was established to relate cell death rate to the shear environment. A good correlation between cell death kinetics and the SSF parameter was observed in the verification test in a 15 L STR bioreactor. SSF can be used to determine the maximum operating range of hydrodynamic stress, and improve the design and optimization of the of C. tinctorius L. cultivation in scale-up process.