Enhanced hypericin extraction from Hypericum perforatum L. by coupling microwave with enzyme-assisted strategy

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Enhanced hypericin extraction from Hypericum perforatum L. by coupling microwave with enzyme-assisted strategy

Journal Article (2019)

Author(s)

Jie Zhang (Chongqing Technology and Business University)

Chunrong Feng (Chongqing Technology and Business University)

Hui Xu (Chongqing Technology and Business University)

Xuemei Tan (Chongqing Technology and Business University)

Peter Leon Hagedoorn (TU Delft - BT/Biocatalysis)

Sheguang Ding (Chongqing Technology and Business University)

Research Group

BT/Biocatalysis

Enzyme Microwave Hypericin Hypericum perforatum L. Xylanase

DOI related publication

https://doi.org/10.1016/j.indcrop.2019.05.036 Final published version

To reference this document use

https://resolver.tudelft.nl/uuid:fa1f0f79-199b-4183-adb2-0676bb605fa5

More Info

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Publication Year

2019

Language

English

Research Group

BT/Biocatalysis

Journal title

Industrial Crops and Products

Volume number

137

Pages (from-to)

231-238

Downloads counter

218

Abstract

Hypericin is considered to be the most biologically active substance in the crude extract of Hypericum perforatum L. (also known as St. John's wort) and has a wide range of pharmacological effects. In this study, a high resolution high performance liquid chromatography method for determining hypericin was established by comparing different chromatographic conditions. Xylanase-assisted extraction and microwave-assisted extraction can improve the extraction yield of hypericin significantly. And the coupling strategy between two methods resulted in a significant difference on the extraction yield. Microwave-assisted extraction after xylanase-assisted extraction was found to be the most efficient strategy for extracting hypericin. The yield was 0.319 ± 0.006 mg g
⁻¹
, which was a 209.7% increase over unassisted extraction. The combination of enzyme assisted extraction followed by microwave assisted extraction can be more commonly applied to improve extraction efficiency of bioactive compounds from plants.