Enhanced hypericin extraction from Hypericum perforatum L. by coupling microwave with enzyme-assisted strategy

Journal article (2019)

Authors

Jie Zhang Chongqing Technology and Business University
Chunrong Feng Chongqing Technology and Business University
Hui Xu Chongqing Technology and Business University
Xuemei Tan Chongqing Technology and Business University
P.L. Hagedoorn BT/Biocatalysis - AS
Sheguang Ding Chongqing Technology and Business University
Research Group
BT/Biocatalysis (AS) (TU Delft)
Enzyme Microwave Hypericin Hypericum perforatum L. Xylanase
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Published Date

2019

Language

English

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Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract


Hypericin is considered to be the most biologically active substance in the crude extract of Hypericum perforatum L. (also known as St. John's wort) and has a wide range of pharmacological effects. In this study, a high resolution high performance liquid chromatography method for determining hypericin was established by comparing different chromatographic conditions. Xylanase-assisted extraction and microwave-assisted extraction can improve the extraction yield of hypericin significantly. And the coupling strategy between two methods resulted in a significant difference on the extraction yield. Microwave-assisted extraction after xylanase-assisted extraction was found to be the most efficient strategy for extracting hypericin. The yield was 0.319 ± 0.006 mg g
−1
, which was a 209.7% increase over unassisted extraction. The combination of enzyme assisted extraction followed by microwave assisted extraction can be more commonly applied to improve extraction efficiency of bioactive compounds from plants.