A photodecarboxylase from Micractinium conductrix active on medium and short-chain fatty acids
Yunjian Ma (Macau University of Science and Technology, South China University of Technology)
Xuanru Zhong (South China University of Technology)
Bin Wu (South China University of Technology)
Dongming Lan (South China University of Technology)
Hao Zhang (South China University of Technology)
Frank Hollmann (TU Delft - BT/Biocatalysis)
Yonghua Wang (Guangdong Youmei Institute of Intelligent Bio-manufacturing Co, Foshan, South China University of Technology)
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Abstract
Hydrocarbons are essential base chemicals as energy carriers and starting materials for chemical manufacture. So-called fatty acid photodecarboxylases (FAPs) represent interesting catalysts for the conversion of natural fatty acids into hydrocarbons thereby giving access to alkanes from renewable feedstock. Today, however, only few FAPs are known. In the current study we report a new FAP from the marine organism Micractinium conductrix (McFAP). In contrast to currently known FAPs McFAP exhibits high catalytic activity towards short and medium fatty acids. Recombinant expression and basic biochemical characterisation of this new member of the FAP family is reported.