Brandaris 128 ultra-high-speed imaging facility: 10 years of operation, updates, and enhanced features
Erik C. Gelderblom (MIRA Institute for Biomedical Technology and Technical Medicine)
Hendrik J. Vos (Erasmus MC)
Frits Mastik (Erasmus MC)
Telli Faez (Erasmus MC)
Ying Luan (Erasmus MC)
Tom J. A. Kokhuis (Erasmus MC)
Antonius F. W. van der Steen (Erasmus MC)
Detlef Lohse (MIRA Institute for Biomedical Technology and Technical Medicine)
Nico de Jong (Erasmus MC, MIRA Institute for Biomedical Technology and Technical Medicine)
Michel Versluis (MIRA Institute for Biomedical Technology and Technical Medicine)
More Info
expand_more
Abstract
The Brandaris 128 ultra-high-speed imaging facility has been updated over the last 10 years through modifications made to the camera’s hardware and software. At its introduction the camera was able to record 6 sequences of 128 images (500×292 pixels) at a maximum frame rate of 25 Mfps. The segmented mode of the camera was revised to allow for subdivision of the 128 image sensors intoarbitrary segments (1–128) with an inter-segment time of 17 μs. Furthermore, a region of interest can be selected to increase the number of recordings within a single run of the camera from 6 up to 125.By extending the imaging system with a laser-induced fluorescence setup, time-resolved ultra-highspeed fluorescence imaging of microscopic objects has been enabled. Minor updates to the systemare also reported here. © 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4758783]