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Ji, Encheng (author), Leijsten, Lieke (author), Witte-Bouma, Janneke (author), Rouchon, Adelin (author), Di Maggio, Nunzia (author), Banfi, Andrea (author), van Osch, G.J.V.M. (author), Farrell, Eric (author), Lolli, Andrea (author)Tissue engineering bone via endochondral ossification requires the generation of a cartilage template which undergoes vascularisation and remodelling. While this is a promising route for bone repair, achieving effective cartilage vascularisation remains a challenge. Here, we investigated how mineralisation of tissue-engineered cartilage...journal article 2023
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Yang, Sora (author), Klughammer, N. (author), Barth, A. (author), Tanenbaum, M. (author), Dekker, C. (author)Single-molecule fluorescence imaging experiments generally require sub-nanomolar protein concentrations to isolate single protein molecules, which makes such experiments challenging in live cells due to high intracellular protein concentrations. Here, we show that single-molecule observations can be achieved in live cells through a drastic...journal article 2023
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Geelhoed, Jeanine S. (author), Thorup, Casper A. (author), Bjerg, Jesper J. (author), Schreiber, Lars (author), Nielsen, Lars Peter (author), Schramm, Andreas (author), Meysman, F.J.R. (author), Marshall, Ian P.G. (author)Bacterial cells can vary greatly in size, from a few hundred nanometers to hundreds of micrometers in diameter. Filamentous cable bacteria also display substantial size differences, with filament diameters ranging from 0.4 to 8 µm. We analyzed the genomes of cable bacterium filaments from 11 coastal environments of which the resulting 23 new...journal article 2023
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Henzel, J. (author), Bakker, N.J. (author), Najafi, Mehrdad (author), Zardetto, Valerio (author), Veenstra, Sjoerd (author), Isabella, O. (author), Mazzarella, L. (author), Weeber, A.W. (author), Theelen, Mirjam (author)Nonequal current generation in the cells of a photovoltaic module, e.g., due to partial shading, leads to operation in reverse bias. This quickly causes a significant efficiency loss in perovskite solar cells. We report a more quantitative investigation of the reverse bias degradation. Various small reverse biases (negative voltages) were...journal article 2023
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Husseini, Abbas Ali (author), Yazdani, Ali Mohammad (author), Ghadiri, Fatemeh (author), Sisman, A.S. (author)We developed a microchip device using surface acoustic waves (SAW) and sharp-edge glass microparticles to rapidly lyse low-level cell samples. This microchip features a 13-finger pair interdigital transducer (IDT) with a 30-degree focused angle, creating high-intensity acoustic beams converging 6 mm away at a 16 MHz frequency. Cell lysis is...journal article 2023
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Polat, S. (author), Kortlever, R. (author), Eral, H.B. (author)Electrochemical carbon dioxide (CO<sub>2</sub>) reduction is a promising route to convert intermittent renewable energy into fuels and valuable chemical products. Separation of CO<sub>2</sub> reduction products by ion-selective electrochemical technology may play a decisive role in the pursuit of commercially viable CO<sub>2</sub> reduction...journal article 2023
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- Velot, Émilie (author), Balmayor, Elizabeth R. (author), Bertoni, Lélia (author), Chubinskaya, Susan (author), Cicuttini, Flavia (author), de Girolamo, Laura (author), Demoor, Magali (author), van Osch, G.J.V.M. (author), Cucchiarini, Magali (author) review 2023
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Savenije, T.J. (author), Zhao, J. (author), Caselli, V.M. (author)Perovskite-based solar cells have been rapidly developed, with record power conversion efficiencies now exceeding 25%. In order to rationally improve the efficiency of these devices, it is important to understand and quantify the dynamics of the excess charge carriers.book chapter 2023
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Abil, Z. (author), Restrepo Sierra, A.M. (author), Danelon, C.J.A. (author)In cell-free gene expression, low input DNA concentration severely limits the phenotypic output, which may impair in vitro protein evolution efforts. We address this challenge by developing CADGE, a strategy that is based on clonal isothermal amplification of a linear gene-encoding dsDNA template by the minimal Φ29 replication machinery and...journal article 2023
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Stoter, Stein K.F. (author), Divi, Sai C. (author), van Brummelen, E. Harald (author), Larson, Mats G. (author), de Prenter, Frits (author), Verhoosel, Clemens V. (author)In this article, we study the effect of small-cut elements on the critical time-step size in an immersogeometric explicit dynamics context. We analyze different formulations for second-order (membrane) and fourth-order (shell-type) equations, and derive scaling relations between the critical time-step size and the cut-element size for various...journal article 2023
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Kapetanović, M. (author), Nunez, Alfredo (author), van Oort, N. (author), Goverde, R.M.P. (author)Hydrogen fuel cell multiple unit vehicles are acquiring a central role in the transition process towards carbon neutral trains operation in non-electrified regional railway networks. In addition to their primary role as a transport mean, these vehicles offer significant potential for applications in innovative concepts such as smart grids....abstract 2023
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Bhadra, A. (author), Haverkort, J.W. (author)Microfluidic fuel cells, electrolyzers, and redox flow batteries utilize laminar flow channels to provide reactants, remove products and avoid their crossover. These devices often also employ porous flow-through electrodes as they offer a high surface area for the reaction and excellent mass transfer. The geometrical features of these...journal article 2023
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Disela, R.C. (author), Bussy, Olivier Le (author), Geldhof, Geoffroy (author), Pabst, Martin (author), Ottens, M. (author)Mass-spectrometry-based proteomics is increasingly employed to monitor purification processes or to detect critical host cell proteins in the final drug substance. This approach is inherently unbiased and can be used to identify individual host cell proteins without prior knowledge. In process development for the purification of new...journal article 2023
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Chen, N. (author), Tune, Daniel (author), Buchholz, Florian (author), Roescu, Razvan (author), Zeman, M. (author), Isabella, O. (author), Mihailetchi, Valentin D. (author)In this study, the edge passivation effectiveness and long-term stability of Nafion polymer in n-type interdigitated back contact (IBC) solar cells are investigated. For new module technologies such as half-cut, triple-cut, or shingled modules, cutting of the cells introduces unpassivated edges with a high recombination rate and this limits the...journal article 2023
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Yin, Xiaole (author), Chen, Xi (author), Jiang, Xiao Tao (author), Yang, Ying (author), Li, Bing (author), Shum, Marcus Ho Hin (author), Schmitt, H. (author), Cha, Chang Jun (author), van Loosdrecht, Mark C.M. (author)Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys in human and animal sectors under the "One-Health"framework. However, there are substantial challenges in comparing and synthesizing the results of multiple studies that employ different test methods and...journal article 2023
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Zhao, Y. (author), Datta, Kunal (author), Paggiaro, Giulia (author), Liu, Hanchen (author), Fardousi, Mohua (author), Santbergen, R. (author), Procel Moya, P.A. (author), Han, C. (author), Yang, G. (author), Weeber, A.W. (author), Zeman, M. (author), Mazzarella, L. (author), Isabella, O. (author)Monolithic perovskite/c-Si tandem solar cells have attracted enormous research attention and have achieved efficiencies above 30%. This work describes the development of monolithic tandem solar cells based on silicon heterojunction (SHJ) bottom- and perovskite top-cells and highlights light management techniques assisted by optical simulation...journal article 2023
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Jiang, Bojing (author), Tan, Sin Yen (author), Fang, Shiyu (author), Feng, Xiaohan (author), Park, B.P. (author), Fok, Hong Kiu Francis (author), Yang, Zhongguang (author), Wang, Ri (author), Kou, Songzi (author), Wu, Angela Ruohao (author), Sun, Fei (author)Spider silk is recognized for its exceptional mechanical properties and biocompatibility, making it a versatile platform for developing functional materials. In this study, a modular functionalization strategy for recombinant spider silk is presented using SpyTag/SpyCatcher chemistry, a prototype of genetically encoded click chemistry. The...journal article 2023
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Magdanz, Veronika (author), Klingner, Anke (author), Abelmann, L. (author), Khalil, Islam S.M. (author)Cell membrane potential affects the electrostatic self-assembly of magnetizable nanoparticles around the flagellum of sperm cells, leading to the formation of biohybrid microrobots (i.e., IRONSperm) with various bending stiffness. Here we explain the influence of bull sperm cell membrane potential on the formation of two types of IRONSperm...journal article 2023
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Yarali, E. (author), Zadpoor, A.A. (author), Staufer, U. (author), Accardo, A. (author), Mirzaali, Mohammad J. (author)Mechanical and morphological design parameters, such as stiffness or porosity, play important roles in creating orthopedic implants and bone substitutes. However, we have only a limited understanding of how the microarchitecture of porous scaffolds contributes to bone regeneration. Meta-biomaterials are increasingly used to precisely engineer...review 2023
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Novella-Rausell, Claudio (author), Grudniewska, Magda (author), Peters, Dorien J.M. (author), Mahfouz, A.M.E.T.A. (author)The kidney's cellular diversity is on par with its physiological intricacy; yet identifying cell populations and their markers remains challenging. Here, we created a comprehensive atlas of the healthy adult mouse kidney (MKA: Mouse Kidney Atlas) by integrating 140.000 cells and nuclei from 59 publicly available single-cell and single-nuclei...journal article 2023