Print Email Facebook Twitter A new semi-orthotopic bone defect model for cell and biomaterial testing in regenerative medicine Title A new semi-orthotopic bone defect model for cell and biomaterial testing in regenerative medicine Author Andrés Sastre, E. (Erasmus MC) Nossin, Y. (Erasmus MC) Jansen, I. (Erasmus MC; Eindhoven University of Technology) Kops, N. (Erasmus MC) Intini, C. (Royal College of Surgeons in Ireland) Witte-Bouma, J. (Erasmus MC) van Rietbergen, B. (Eindhoven University of Technology; Maastricht UMC) Hofmann, S. (Eindhoven University of Technology) van Osch, G.J.V.M. (TU Delft Biomaterials & Tissue Biomechanics; Erasmus MC) Date 2021 Abstract In recent decades, an increasing number of tissue engineered bone grafts have been developed. However, expensive and laborious screenings in vivo are necessary to assess the safety and efficacy of their formulations. Rodents are the first choice for initial in vivo screens but their size limits the dimensions and number of the bone grafts that can be tested in orthotopic locations. Here, we report the development of a refined murine subcutaneous model for semi-orthotopic bone formation that allows the testing of up to four grafts per mouse one order of magnitude greater in volume than currently possible in mice. Crucially, these defects are also "critical size" and unable to heal within the timeframe of the study without intervention. The model is based on four bovine bone implants, ring-shaped, where the bone healing potential of distinct grafts can be evaluated in vivo. In this study we demonstrate that promotion and prevention of ossification can be assessed in our model. For this, we used a semi-automatic algorithm for longitudinal micro-CT image registration followed by histological analyses. Taken together, our data supports that this model is suitable as a platform for the real-time screening of bone formation, and provides the possibility to study bone resorption, osseointegration and vascularisation. Subject Animal modelBoneBone substitutesEndochondral ossificationGuided tissue regenerationTissue scaffolds To reference this document use: http://resolver.tudelft.nl/uuid:0f9b620c-a1e3-4f0b-a5c3-6a4cd88b65c4 DOI https://doi.org/10.1016/j.biomaterials.2021.121187 Source Biomaterials, 279 Part of collection Institutional Repository Document type journal article Rights © 2021 E. Andrés Sastre, Y. Nossin, I. Jansen, N. Kops, C. Intini, J. Witte-Bouma, B. van Rietbergen, S. Hofmann, G.J.V.M. van Osch, More Authors Files PDF 1_s2.0_S0142961221005445_main.pdf 11.93 MB Close viewer /islandora/object/uuid:0f9b620c-a1e3-4f0b-a5c3-6a4cd88b65c4/datastream/OBJ/view