A new optical method for characterizing single molecule interactions based on dark field microscopy

Conference paper (2007)
Department
Quantitative Imaging Group (AS) (TU Delft)
Copyright: © 2007 Dietrich, H.R.C., Vermolen, B.J., Rieger, B., Young, I.T., Garini, Y.
Dark field microscopy Single molecule detection Molecular interactions Gold nanobeads Tethered-praticle motion
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Published Date

10-02-2007

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Source:

Proceedings of SPIE, 2007 vol. 6444

ISSN:

0277-786X

Faculty

Applied Sciences

Department

Quantitative Imaging Group

Abstract

Single-molecule techniques continue to gain in popularity in research disciplines such as the study of intermolecular interactions. These techniques provide information that otherwise would be lost by using bulk measurements that deal with a large number of molecules. We describe in this report the motion of tethered DNA molecules that have been tagged with gold nanobeads and observed under dark field microscopy to study single molecular interactions (SMI). We further report on the derivation and use of several physical parameters and how these parameters change under differing experimental conditions.

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