Title
Natronoglycomyces albus gen. Nov., sp. nov, a haloalkaliphilic actinobacterium from a soda solonchak soil
Author
Sorokin, Dimitry Y. (TU Delft BT/Environmental Biotechnology; TU Delft BT/Biotechnologie; Russian Academy of Sciences) 
Khijniak, Tatjana V. (Russian Academy of Sciences)
Zakharycheva, Alicia P. (Russian Academy of Sciences)
Elcheninov, Alexander G. (Russian Academy of Sciences)
Hahnke, Richard L. (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures)
Boueva, Olga V. (Russian Academy of Sciences)
Ariskina, Elena V. (Russian Academy of Sciences)
Bunk, Boyke (Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures)
Kublanov, Ilya V. (Russian Academy of Sciences)
Evtushenko, Lyudmila I. (Russian Academy of Sciences)
Department
BT/Biotechnologie
Date
2021
Abstract
A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia. The isolate was facultatively alkaliphilic, growing at pH 6.5–10.5 (optimum at 7.3–9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates. The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome. The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1%) and Salininema pro-teolyticum Miq-4T (91.8%). The average amino acid identity values (56.1–61.5%) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera. The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'. The whole-cell sugars included mannose, galactose, arabinose, ribose and xylose. The major menaquinones were MK-10(Н4) and MK-11(Н4). The identified polar lipids were represented by phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylg-lycerol, phosphatidylinositol and phosphatidylinositol mannosides. In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol. The polar lipid fatty acids were dominated by anteiso-C17:0 and iso-C16:0. The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp. Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp. nov, is proposed with the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T).
Subject
Glycomycetaceae
Haloalkaliphilic
Hydrolytic
Natronoglycomyces
Soda solonchak soil
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http://resolver.tudelft.nl/uuid:12fa7238-ec77-4048-a7c0-257c15f45fa3
DOI
https://doi.org/10.1099/ijsem.0.004804
ISSN
1466-5026
Source
International Journal of Systematic and Evolutionary Microbiology, 71 (5)
Part of collection
Institutional Repository
Document type
journal article
Rights
© 2021 Dimitry Y. Sorokin, Tatjana V. Khijniak, Alicia P. Zakharycheva, Alexander G. Elcheninov, Richard L. Hahnke, Olga V. Boueva, Elena V. Ariskina, Boyke Bunk, Ilya V. Kublanov, Lyudmila I. Evtushenko