Print Email Facebook Twitter Bacterial chromosome organization by ParB proteins Title Bacterial chromosome organization by ParB proteins Author Tišma, M. (TU Delft BN/Cees Dekker Lab) Contributor Dekker, C. (promotor) Dogterom, A.M. (promotor) Degree granting institution Delft University of Technology Date 2024-03-21 Abstract This thesis explores the mechanisms that underlie chromosome organization in bacteria. Bacteria are considered amongst the simplest living organisms on our planet. They lack the cellular organization found in other domains of life (Archaea or Eukaryotics) and often have simpler life cycles. Over the past decade, we gained increasing knowledge pointing to the fact that bacteria allocate a lot of resources to precisely organize their genome within the cell, and to segregate two genomes after DNA replication to daughter cells.In this thesis, I investigated DNA organization and segregation systems in a model system bacterium Bacillus subtilis. I approached this feat both from the in vivo aspect – imaging in a live bacterium, and from the in vitro aspect – observing isolated proteins and DNA molecules. This holistic approach allowed me to gain deep insight into the proteins and mechanisms needed for DNA organization and segregation.... Subject Single-molecule BiophysicsSingle-molecule fluorescenceIn vitro AssaysMagnetic TweezersParABSChromosome SegregationParB ProteinSupercoilingDNA DyesParB-ParB RecruitmentDNA CondensationLive Cell Imaging To reference this document use: https://doi.org/10.4233/uuid:19906f66-bca0-46e6-9af8-584a8ee0a73c Series Casimir PhD Series, Delft-Leiden, 2023-30 Part of collection Institutional Repository Document type doctoral thesis Rights © 2024 M. Tišma Files PDF Full_Thesis_MTisma.pdf 33.74 MB Close viewer /islandora/object/uuid:19906f66-bca0-46e6-9af8-584a8ee0a73c/datastream/OBJ/view