Title
Extracellular and intracellular effects of bioactive glass nanoparticles on osteogenic differentiation of bone marrow mesenchymal stem cells and bone regeneration in zebrafish osteoporosis model
Author
Meng, Li (Nanjing Medical University)
Zhao, Panpan (Nanjing Medical University)
Jiang, Yucheng (Nanjing Medical University)
You, Jiawen (Nanjing Medical University)
Xu, Zhiyan (Friedrich-Alexander-Universität Erlangen-Nürnberg)
Yu, K. (TU Delft BN/Marie-Eve Aubin-Tam Lab) 
Boccaccini, Aldo R. (Friedrich-Alexander-Universität Erlangen-Nürnberg)
Ma, Junqing (Nanjing Medical University)
Zheng, K. (Nanjing Medical University)
Date
2024
Abstract
Bioactive glass nanoparticles (BGNs) are well-recognized multifunctional biomaterials for bone tissue regeneration due to their capability to stimulate various cellular processes through released biologically active ions. Understanding the correlation between BGN composition and cellular responses is key to developing clinically usable BGN-based medical devices. This study investigated the influence of CaO content of binary SiO2-CaO BGNs (CaO ranging from 0 to 10 mol%) on osteogenic differentiation of rat bone marrow mesenchymal stem cells (rBMSCs) and in vivo bone regeneration in zebrafish osteoporosis model. The results showed that BGNs could promote osteogenic differentiation of rBMSCs by indirectly releasing active ions or directly interacting with rBMSCs by internalization. In both situations, BGNs of a higher CaO content could promote the osteogenic differentiation of rBMSCs to a greater extent. The internalized BGNs could activate the transcription factors RUNX2 and OSX, leading to the expression of osteogenesis-related genes. The results in the zebrafish osteoporosis model indicated that the presence of BGNs of higher CaO contents could enhance bone regeneration and rescue dexamethasone-induced osteoporosis to a greater extent. These findings demonstrate that BGNs can stimulate osteogenic differentiation of rBMSCs by releasing active ions or internalization. A higher CaO content facilitates osteogenesis and bone regeneration of zebrafish as well as relieving dexamethasone-induced osteoporosis. The zebrafish osteoporosis model can be a potent tool for evaluating the in vivo bone regeneration effects of bioactive materials. Statement of significance: Bioactive glass nanoparticles (BGNs) are increasingly used as fillers of nanocomposites or as delivery platforms of active ions to regenerate bone tissue. Various studies have shown that BGNs can enhance osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) by releasing active ions. However, the correlation between BGN composition and cellular responses and in vivo bone regeneration effect has still not been well investigated. Establishment of a suitable in vivo animal model for investigating this correlation is also challenging. The present study reports the influence of CaO content in binary SiO2-CaO BGNs on osteogenic differentiation of BMSCs extracellularly and intracellularly. This study also demonstrates the suitability of zebrafish osteoporosis model to investigate in vivo bone regeneration effect of BGNs.
Subject
Bioactive glass
Bone regeneration
Cellular uptake
Osteoporosis
Zebrafish model
To reference this document use:
http://resolver.tudelft.nl/uuid:34719c39-48f2-4edb-9e8d-1fc8af48996a
DOI
https://doi.org/10.1016/j.actbio.2023.11.037
Embargo date
2024-07-15
ISSN
1742-7061
Source
Acta Biomaterialia, 174, 412-427
Bibliographical note
Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.
Part of collection
Institutional Repository
Document type
journal article
Rights
© 2024 Li Meng, Panpan Zhao, Yucheng Jiang, Jiawen You, Zhiyan Xu, K. Yu, Aldo R. Boccaccini, Junqing Ma, K. Zheng