Print Email Facebook Twitter Low-cost fluorescence microscope with microfluidic device fabrication for optofluidic applications Title Low-cost fluorescence microscope with microfluidic device fabrication for optofluidic applications Author Nagalingam, Nagaraj (TU Delft Complex Fluid Processing) Raghunathan, Aswin (Student TU Delft) Korede, V.B. (TU Delft Complex Fluid Processing) Overmars, E.F.J. (TU Delft Fluid Mechanics) Hung, S. (TU Delft Team Carlas Smith) Hartkamp, Remco (TU Delft Complex Fluid Processing) Padding, J.T. (TU Delft Complex Fluid Processing) Smith, C.S. (TU Delft Team Carlas Smith) Eral, H.B. (TU Delft Complex Fluid Processing) Date 2023 Abstract Optofluidic devices have revolutionized the manipulation and transportation of fluid at smaller length scales ranging from micrometers to millimeters. We describe a dedicated optical setup for studying laser-induced cavitation inside a microchannel. In a typical experiment, we use a tightly focused laser beam to locally evaporate the solution laced with a dye resulting in the formation of a microbubble. The evolving bubble interface is tracked using high-speed microscopy and digital image analysis. Furthermore, we extend this system to analyze fluid flow through fluorescence-Particle Image Velocimetry (PIV) technique with minimal adaptations. In addition, we demonstrate the protocols for the in-house fabrication of a microchannel tailored to function as a sample holder in this optical setup. In essence, we present a complete guide for constructing a fluorescence microscope from scratch using standard optical components with flexibility in the design and at a lower cost compared to its commercial analogues. Subject MicrofluidicsExperimentsLaser-induced cavitationFluorescence microscopyHigh-speed imaging To reference this document use: http://resolver.tudelft.nl/uuid:48750d98-022d-4a97-8b92-458a803e4460 DOI https://doi.org/10.1016/j.ohx.2023.e00415 ISSN 2468-0672 Source HardwareX, 14 Part of collection Institutional Repository Document type journal article Rights © 2023 Nagaraj Nagalingam, Aswin Raghunathan, V.B. Korede, E.F.J. Overmars, S. Hung, Remco Hartkamp, J.T. Padding, C.S. Smith, H.B. Eral Files PDF 1_s2.0_S2468067223000226_main.pdf 3.19 MB Close viewer /islandora/object/uuid:48750d98-022d-4a97-8b92-458a803e4460/datastream/OBJ/view