Self-assembly of ammonium assimilation microbiomes regulated by COD/N ratio

Self-assembly of ammonium assimilation microbiomes regulated by COD/N ratio

Han, Fei (Shandong University)
Zhang, Mengru (Shandong University)
Li, Zhe (Shandong University)
Liu, Zhe (School of Environmental Science and Engineering; Shandong University)
Han, Yufei (Shandong University)
Li, L. (TU Delft Sanitary Engineering)
Zhou, Weizhi (Shandong University)

2023

Marine microorganisms have an inherent advantage in the treatment of saline wastewater due to their halophilic properties. Ammonium assimilation is the most important and common nitrogen conversion pathway in the ocean, which means that it may be a suitable nitrogen removal strategy under high salinity conditions. However, the targeted construction of engineering microbiomes with ammonium assimilation function for nitrogen recovery has not been realized. Here, we constructed four halophilic ammonium assimilation microbiomes from marine microbial community under varying chemical oxygen demand (COD) to nitrogen (COD/N) ratios. The regulation of COD/N ratio on microbial self-assembly was explored at the phenotypic, genetic, and microbial levels. The results of nitrogen balance tests, functional genes abundance and microbial community structure confirmed that the microbiomes regulated by different COD/N ratios all performed obligate ammonium assimilation functions. >93% of ammonium, 90% of TN, 98% of COD, and 82% of phosphorus were simultaneously removed by microbial assimilation under the COD/N ratio of 20. COD/N ratios significantly affected the self-assembly of microbiomes by selectively enriching heterotrophic microorganisms with different preference for organic carbon load. Additionally, the increase of COD/N ratio intensified the competition among species within the microbiome (the proportion of negative connections of microbial network increased from 5.0% to 24.4%), which may enhance the stability of community structure. Taken together, these findings can provide theoretical guidance for the construction and optimization of engineering microbiomes for synergistic nitrogen removal and recovery.

Ammonium assimilation microbiomes
Chemical oxygen demand to nitrogen (COD/N) ratios
Marine microbial community
Microbial network
Self-assembly process

http://resolver.tudelft.nl/uuid:4cf4c51a-6995-4fb3-bb07-2803625aa802

https://doi.org/10.1016/j.cej.2022.140782

2023-07-01

1385-8947

Chemical Engineering Journal, 455

Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.

Institutional Repository

journal article

© 2023 Fei Han, Mengru Zhang, Zhe Li, Zhe Liu, Yufei Han, L. Li, Weizhi Zhou