Triplet–triplet annihilation-based photon-upconversion to broaden the wavelength spectrum for photobiocatalysis

Triplet–triplet annihilation-based photon-upconversion to broaden the wavelength spectrum for photobiocatalysis

Hwang, Se Yeun (Ewha Womans University)
Song, Dayoon (Ewha Womans University)
Seo, Eun Ji (Ewha Womans University)
Hollmann, F. (TU Delft BT/Biocatalysis)
You, Youngmin (Ewha Womans University)
Park, Jin Byung (Ewha Womans University)

2022

Photobiocatalysis is a growing field of biocatalysis. Especially light-driven enzyme catalysis has contributed significantly to expanding the scope of synthetic organic chemistry. However, photoenzymes usually utilise a rather narrow wavelength range of visible (sun)light. Triplet–triplet annihilation-based upconversion (TTA-UC) of long wavelength light to shorter wavelength light may broaden the wavelength range. To demonstrate the feasibility of light upconversion we prepared TTA-UC poly(styrene) (PS) nanoparticles doped with platinum(II) octaethylporphyrin (PtOEP) photosensitizer and 9,10-diphenylanthracene (DPA) annihilator (PtOEP:DPA@PS) for application in aqueous solutions. Photoexcitation of PtOEP:DPA@PS nanoparticles with 550 nm light led to upconverted emission of DPA 418 nm. The TTA-UC emission could photoactivate flavin-dependent photodecarboxylases with a high energy transfer efficiency. This allowed the photodecarboxylase from Chlorella variabilis NC64A to catalyse the decarboxylation of fatty acids into long chain secondary alcohols under green light (λ = 550 nm).

http://resolver.tudelft.nl/uuid:6747842e-54d2-405e-aaf6-4c2c8c63281c

https://doi.org/10.1038/s41598-022-13406-8

2045-2322

Scientific Reports, 12 (1)

Institutional Repository

journal article

© 2022 Se Yeun Hwang, Dayoon Song, Eun Ji Seo, F. Hollmann, Youngmin You, Jin Byung Park