Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production

Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production

van Aalst, A.C.A. (TU Delft BT/Industriele Microbiologie)
de Valk, S.C. (TU Delft BT/Industriele Microbiologie)
van Gulik, W.M. (TU Delft BT/Industriele Microbiologie)
Jansen, Mickel L.A. (DSM)
Pronk, J.T. (TU Delft BT/Biotechnologie)
Mans, R. (TU Delft BT/Industriele Microbiologie)

BT/Biotechnologie

2022

Product yield on carbohydrate feedstocks is a key performance indicator for industrial ethanol production with the yeast Saccharomyces cerevisiae. This paper reviews pathway engineering strategies for improving ethanol yield on glucose and/or sucrose in anaerobic cultures of this yeast by altering the ratio of ethanol production, yeast growth and glycerol formation. Particular attention is paid to strategies aimed at altering energy coupling of alcoholic fermentation and to strategies for altering redox-cofactor coupling in carbon and nitrogen metabolism that aim to reduce or eliminate the role of glycerol formation in anaerobic redox metabolism. In addition to providing an overview of scientific advances we discuss context dependency, theoretical impact and potential for industrial application of different proposed and developed strategies.

Biofuels
Energy metabolism
Metabolic engineering
Redox metabolism
Saccharomyces cerevisiae
Synthetic biology

http://resolver.tudelft.nl/uuid:719f0bdd-932b-431f-8b60-0b8504c8426a

https://doi.org/10.1016/j.synbio.2021.12.010

Synthetic and Systems Biotechnology, 7 (1), 554-566

Institutional Repository

journal article

© 2022 A.C.A. van Aalst, S.C. de Valk, W.M. van Gulik, Mickel L.A. Jansen, J.T. Pronk, R. Mans