Print Email Facebook Twitter Influence of humic substances created in THP on partial nitritation step of PN/A reactors Title Influence of humic substances created in THP on partial nitritation step of PN/A reactors Author Wang, Sihan (TU Delft Civil Engineering & Geosciences) Contributor de Kreuk, M.K. (mentor) van Loosdrecht, Mark C.M. (graduation committee) Pavez Jara, J.A. (graduation committee) Degree granting institution Delft University of Technology Programme Civil Engineering | Environmental Engineering Date 2022-09-26 Abstract Anaerobic digestion (AD) is an effective and economical in secondary sludge management. Thermal hydrolysis process (THP) is a reliable pre-treatment method to improve the biodegradability of sludge. Partial nitritation and anammox process (PN/A) is a suitable method used to remove nitrogen in reject water which has a low COD/N ratio. However, some organic compounds produced in THP process, such as melanoidins, have negative effects on AOO and anammox bacteria. Considering that humic substances occupy a large part of the soluble organics in reject water of THP-AD process. Therefore, this study aimed to investigate the acute and long-term toxicity of three types of humic substances (commercial humic acid, melanoidins and reject water) on partial nitritation step of PN/A process. The biodegradability of three types of humic substances under aerobic and anaerobic conditions, and the evolution of humic substances during the biodegradability assays were also analysed. The results showed that all three types of humic substances had higher biodegradability under aerobic condition than that under aerobic condition, and melanoidins had more biodegradable compounds than reject water and humic acid. During the AD process, humic substances acted as electron acceptors, decreasing the cumulative methane production of all three types of humic substances compared to the blank. Also, small MW fractions were transformed to large MW fractions in all three types of humic substances during both aerobic and anaerobic digestion. Moreover, during aerobic and AD, both fulvic acid-like and humic acid-like substances were formed. In addition, the results of acute toxicity tests showed commercial humic acids had a stronger inhibitory effect than reject water and melanoidins on AOO biomass. In addition, a positive correlation between humic substances concentration and AOO activity loss was observed. The long-term operation of PN reactor was failed due to the formation of granular sludge in the reactor and the growth of AOO biomass in the tubing and culture medium. Subject Thermal hydrolysis processPartial nitritation/AnammoxHumic substancesBiodegradabilityInhibition To reference this document use: http://resolver.tudelft.nl/uuid:7575e7f0-3c7a-401e-924b-c1b25fc06064 Embargo date 2023-09-30 Part of collection Student theses Document type master thesis Rights © 2022 Sihan Wang Files PDF Sihan_s_thesis_reprt_final.pdf 7.95 MB Close viewer /islandora/object/uuid:7575e7f0-3c7a-401e-924b-c1b25fc06064/datastream/OBJ/view