Print Email Facebook Twitter Comparison of acute phase response in mice after inhalation and intratracheal instillation of molybdenum disulphide and tungsten particles Title Comparison of acute phase response in mice after inhalation and intratracheal instillation of molybdenum disulphide and tungsten particles Author Gutierrez, Claudia Torero (University of Copenhagen; National Research Centre for the Working Environment) Loizides, Charis (The Cyprus Insitute) Hafez, Iosif (The Cyprus Insitute) Biskos, G. (TU Delft Atmospheric Remote Sensing; The Cyprus Insitute) Loeschner, Katrin (Technical University of Denmark) Brostrøm, Anders (National Research Centre for the Working Environment) Roursgaard, Martin (University of Copenhagen) Saber, Anne Thoustrup (National Research Centre for the Working Environment) Møller, Peter (University of Copenhagen) Date 2023 Abstract Inhalation studies are the gold standard for assessing the toxicity of airborne materials. They require considerable time, special equipment, and large amounts of test material. Intratracheal instillation is considered a screening and hazard assessment tool as it is simple, quick, allows control of the applied dose, and requires less test material. The particle-induced pulmonary inflammation and acute phase response in mice caused by intratracheal instillation or inhalation of molybdenum disulphide or tungsten particles were compared. End points included neutrophil numbers in bronchoalveolar lavage fluid, Saa3 mRNA levels in lung tissue and Saa1 mRNA levels in liver tissue, and SAA3 plasma protein. Acute phase response was used as a biomarker for the risk of cardiovascular disease. Intratracheal instillation of molybdenum disulphide or tungsten particles did not produce pulmonary inflammation, while molybdenum disulphide particles induced pulmonary acute phase response with both exposure methods and systemic acute phase response after intratracheal instillation. Inhalation and intratracheal instillation showed similar dose–response relationships for pulmonary and systemic acute phase response when molybdenum disulphide was expressed as dosed surface area. Both exposure methods showed similar responses for molybdenum disulphide and tungsten, suggesting that intratracheal instillation can be used for screening particle-induced acute phase response and thereby particle-induced cardiovascular disease. Subject acute phase responseinhalationintratracheal instillationmolybdenum disulphidetungsten To reference this document use: http://resolver.tudelft.nl/uuid:8192575b-83a9-4cdd-aa2f-4e68e26265bc DOI https://doi.org/10.1111/bcpt.13915 ISSN 1742-7835 Source Basic and Clinical Pharmacology and Toxicology, 133 (3), 265-278 Part of collection Institutional Repository Document type journal article Rights © 2023 Claudia Torero Gutierrez, Charis Loizides, Iosif Hafez, G. Biskos, Katrin Loeschner, Anders Brostrøm, Martin Roursgaard, Anne Thoustrup Saber, Peter Møller, More Authors Files PDF Basic_Clin_Pharma_Tox_202 ... acheal.pdf 1.45 MB Close viewer /islandora/object/uuid:8192575b-83a9-4cdd-aa2f-4e68e26265bc/datastream/OBJ/view