Studying genomic processes at the single-molecule level

introducing the tools and applications

Journal article (2013)

Authors

D. Dulin BN/Nynke Dekker Lab - AS
J. Lipfert BN/Nynke Dekker Lab - AS
M.C. Moolman BN/Nynke Dekker Lab - AS
N.H. Dekker BN/Nynke Dekker Lab - AS
Research Group
BN/Nynke Dekker Lab (AS) (TU Delft)
Copyright: © 2013 D. Dulin, J. Lipfert, M.C. Moolman, N.H. Dekker
DOI: https://doi.org/10.1038/nrg3316
Genomics Technology Gene expression Microbial genetics
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Published Date

2013

Language

English

Reuse Rights

Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.

Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Nynke Dekker Lab

Abstract

To understand genomic processes such as transcription, translation or splicing, we need to be able to study their spatial and temporal organization at the molecular level. Single-molecule approaches provide this opportunity, allowing researchers to monitor molecular conformations, interactions or diffusion quantitatively and in real time in purified systems and in the context of the living cell. This Review introduces the types of application of single-molecule approaches that can enhance our understanding of genome function.