Natronobiforma cellulositropha gen. nov., sp. nov., a novel haloalkaliphilic member of the family Natrialbaceae (class Halobacteria) from hypersaline alkaline lakes

Natronobiforma cellulositropha gen. nov., sp. nov., a novel haloalkaliphilic member of the family Natrialbaceae (class Halobacteria) from hypersaline alkaline lakes

Sorokin, Dimitry Y. (TU Delft BT/Environmental Biotechnology; Russian Academy of Sciences)
Khijniak, Tatiana V. (Russian Academy of Sciences)
Kostrikina, Nadezhda A. (Russian Academy of Sciences)
Elcheninov, Alexander G. (Russian Academy of Sciences)
Toshchakov, Stepan V. (Immanuel Kant Baltic Federal University)
Bale, Nicole J. (Universiteit Utrecht)
Damsté, Jaap S.Sinninghe (Universiteit Utrecht)
Kublanov, Ilya V. (Russian Academy of Sciences; Immanuel Kant Baltic Federal University)

2018

Six strains of extremely halophilic and alkaliphilic euryarchaea were enriched and isolated in pure culture from surface brines and sediments of hypersaline alkaline lakes in various geographical locations with various forms of insoluble cellulose as growth substrate. The cells are mostly flat motile rods with a thin monolayer cell wall while growing on cellobiose. In contrast, the cells growing with cellulose are mostly nonmotile cocci covered with a thick external EPS layer. The isolates, designated AArcel, are obligate aerobic heterotrophs with a narrow substrate spectrum. All strains can use insoluble celluloses, cellobiose, a few soluble glucans and xylan as their carbon and energy source. They are extreme halophiles, growing within the range from 2.5 to 4.8 M total Na⁺ (optimum at 4 M) and obligate alkaliphiles, with the pH range for growth from 7.5 to 9.9 (optimum at 8.5–9). The core archaeal lipids of strain AArcel5^T were dominated by C₂₀–C₂₀ dialkyl glycerol ether (DGE) (i.e. archaeol) and C₂₀–C₂₅ DGE in nearly equal proportion. The 16S rRNA gene analysis indicated that all six isolates belong to a single genomic species mostly related to the genera Saliphagus-Natribaculum-Halovarius. Taking together a substantial phenotypic difference of the new isolates from the closest relatives and the phylogenetic distance, it is concluded that the AArcel group represents a novel genus-level branch within the family Natrialbaceae for which the name Natronobiforma cellulositropha gen. nov., sp. nov. is proposed with AArcel5^T as the type strain (JCM 31939^T = UNIQEM U972^T).

Cellulotrophic
Haloalkaliphilic
Hypersaline
Natronoarchaea
Soda lakes

http://resolver.tudelft.nl/uuid:9acb3b62-d876-42b1-a802-25409fd8d742

https://doi.org/10.1016/j.syapm.2018.04.002

0723-2020

Systematic and Applied Microbiology

doi 10.1016/j.syapm.2018.11.007 contains this corrigendum: 1. The species name “cellulotropha” was corrected to “cellulositropha” in the (SPNA), (SPEP) and TITL lines. 2. The author name “Damstéd” in the (AUT) was corrected to “Damsté”. 3.The word “neutral” in the (GETY/SPTY) was corrected to “neut”. 4.Some of the relevant values have been added to both genus and species columns.

Institutional Repository

journal article

© 2018 Dimitry Y. Sorokin, Tatiana V. Khijniak, Nadezhda A. Kostrikina, Alexander G. Elcheninov, Stepan V. Toshchakov, Nicole J. Bale, Jaap S.Sinninghe Damsté, Ilya V. Kublanov