Print Email Facebook Twitter Fourier Ring Correlation with a single image Title Fourier Ring Correlation with a single image Author ten Brink, Tip (TU Delft Electrical Engineering, Mathematics and Computer Science; TU Delft Applied Sciences) Contributor Rieger, B. (mentor) Visser, P.M. (graduation committee) Degree granting institution Delft University of Technology Programme Applied Mathematics | Applied Physics Date 2022-05-23 Abstract As super-resolution methods make it possible to capture images at a resolution beyond the diffraction limit, they have no straightforward measure for optical resolution. Consequently, signal-to-noise ratio-based methods to determine resolution such as Fourier Ring Correlation (FRC) have seen increaseduse. We look at a new method, called 1FRC, as it requires only a single image instead of two (2FRC, the original method). It splits each pixel value into two values according to a binomial distribution, producing two images usable in a standard FRC routine. We consider for which noise modalities and conditionsthe results are equivalent to using 2FRC. If the image noise is only Poisson-distributed we derive mathematically that 1FRC and 2FRC are equivalent. Using simulations on Siemens star test images we find that the mean squared error between 1FRC and 2FRC curves is small for images containing only Poisson noise and a combination of Poisson and low variance Gaussian noise. However, whenthe mean variance ratio 𝜇/𝜎2 (image mean divided by variance of a pixel) is less than one, the 1FRC curve no longer goes to zero at high frequencies, but instead fluctuates at an elevated level. We see that this elevated 1FRC curve level is exactly 1 − 𝜇/𝜎2. For 𝜇/𝜎2 < 0.973 the difference in resolution exceeds one standard deviation of the 1FRC resolution. From this point the Kolmogorov-Smirnov test confidently states that the 1FRC pixel sum distributions are not equal to 2FRC distributions. We also test 1FRC on an experimental dataset made with varying STED intensity. The computed resolution curves fit well to the modified Abbe equation for STED. However, the 1FRC resolutions are up to 30%better than resolutions obtained using decorrelation analysis. Subject Fourier Ring Correlation1FRCBinomial split To reference this document use: http://resolver.tudelft.nl/uuid:abdc31f6-6ecd-4c1b-a0c4-53711829467a Part of collection Student theses Document type bachelor thesis Rights © 2022 Tip ten Brink Files PDF BEP_1FRC_tmtenbrink_20221505_1.pdf 5.24 MB Close viewer /islandora/object/uuid:abdc31f6-6ecd-4c1b-a0c4-53711829467a/datastream/OBJ/view