BR
Authored
10 records found
Image processing and analysis for single-molecule localization microscopy
Computation for nanoscale imaging
Fluorescence microscopy is currently the most important tool for visualizing biological structures at the subcellular scale. The combination of fluorescence, which enables a high imaging contrast, and the possibility to apply molecular labeling, which allows for a high imaging sp
...
Single‐Molecule Switching
Fluorescence Polarization Control for On–Off Switching of Single Molecules at Cryogenic Temperatures
In article number 1700323, a sparsity‐inducing scheme based on widefield stimulated emission depletion, which requires stringent control of the polarization state of both the excitation and depletion laser, is introduced by Bernd Rieger and co‐workers for superresolution fluoresc
...
ZIMFLUX
Single molecule localization microscopy with patterned illumination in 3D
Three dimensional modulation-enhanced single-molecule localization techniques, such as ModLoc, offer advancements in axial localization precision across the entire field of view and axial capture range, by applying phase shifting to the illumination pattern. However, this improve
...
Super-resolution fight club
Assessment of 2D and 3D single-molecule localization microscopy software
With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for their experiments, we have designe
...
Super-resolution fight club
Assessment of 2D and 3D single-molecule localization microscopy software
With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for their experiments, we have designe
...
Super-resolution fight club
Assessment of 2D and 3D single-molecule localization microscopy software
With the widespread uptake of 2D and 3D single molecule localization microscopy, a large set of different data analysis packages have been developed to generate super-resolution images. To guide researchers on the optimal analytical software for their experiments, we have designe
...
Publisher Correction
Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software (Nature Methods, (2019), 16, 5, (387-395), 10.1038/s41592-019-0364-4)
In the version of this paper originally published, Figure 4a contained errors that were introduced during typesetting. The bottom 11° ThunderSTORM image is an xz view but was incorrectly labeled as xy, and the low x-axis value in the four line profiles was incorrectly set as –60
...
Publisher Correction
Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software (Nature Methods, (2019), 16, 5, (387-395), 10.1038/s41592-019-0364-4)
In the version of this paper originally published, Figure 4a contained errors that were introduced during typesetting. The bottom 11° ThunderSTORM image is an xz view but was incorrectly labeled as xy, and the low x-axis value in the four line profiles was incorrectly set as –60
...
Publisher Correction
3D particle averaging and detection of macromolecular symmetry in localization microscopy (Nature Communications, (2021), 12, 1, (2847), 10.1038/s41467-021-22006-5)
The original HTML version of this Article was updated shortly after publication because the previous HTML version linked to an incorrect Supplementary Information file.
@enLight microscopy, allowing sub-diffraction-limited resolution, has been among the fastest developing techniques at the interface of biology, chemistry, and physics. Intriguingly no theoretical limit exists on how far the underlying measurement uncertainty can be lowered. In part
...
Contributed
10 records found
Population difference between L4 and L5 Trojans due to Jupiter's outward migration
A numerical analysis using actual Trojan data
Ever since their discovery, the Trojans have raised many questions among astrophysicists. In 1989 it was found that there were more L4 than L5 Trojans and the current L4:L5 ratio value is estimated to be 1.6. However, this asymmetry could not be explained by Jupiter’s current orb
...
Application of Green's Functions to Self-Gravitating and Rotating Planets
And Modelling the Gravitational Field of the Earth
A model is designed for solving gravitational profiles of self-gravitating and rotating planets via the use of Poisson's equation for total gravity, i.e., the sum of the gravitational and rotational potential. Poisson's equation is a partial differential equation that is solved w
...
Coopetition in academia
How competition and cooperation can coexist between academic researchers
Academic research is inherently cooperative, with researchers exchanging information through formal publications as well as direct messaging. Academia are, however, becoming increasingly competitive: limited financial resources and research spots, among other things, may now affe
...
Localization microscopy of constrained fluorescent molecules
Pushing towards Ångström-scale resolution through cryogenics
Localization microscopy has circumvented the diffraction limit by sequentially imaging individual light emitting molecules at a time. The position of these individual molecules can be determined and a super-resolution reconstruction is made with improved resolution. Normally free
...
Optimum Single Molecule Localization Microscopy
From Single Molecule Detections to Nanoscopic Observations
Single molecule localization microscopy is a powerful tool that delivers high contrast and imaging specificity at a resolution beyond that of conventional microscopes. To obtain a super-resolved image, one needs to image at least hundred thousand camera frames and estimate the po
...
As super-resolution methods make it possible to capture images at a resolution beyond the diffraction limit, they have no straightforward measure for optical resolution. Consequently, signal-to-noise ratio-based methods to determine resolution such as Fourier Ring Correlation (FR
...
Gravitational waves predicted by Einstein have been measured in a few observatories worldwide. These detectors are complex interferometers with sizes of kilometers. Misalignments and aberrations in their optical set-up can create higher-order modes in the laser beam. These higher
...
For decades, the resolution of fluorescent light microscopy has been bounded by Abbe’s diffraction limit to λ/2NA. Super-resolution methods, awarded with the 2014 Nobel Prize in Chemistry, use tricks to overcome this limit. The general idea is to image blinking fluorophores ...
In 2019 werd er voor het eerst een foto van een zwart gat gepubliceerd. Het betreffende super zware zwarte gat ligt in het midden van het sterrenstelsel Messier 87. In dit onderzoek willen we simulaties maken van hoe een foto van een zwart gat eruit ziet. Zowel voor een achtergro
...
In this thesis new dielectric metasurfaces in immersion were explored with a periodic distribution of unit cells with multiple silicon cylinder structures on a reflective gold slab with a fused quartz spacer in between. These new metasurfaces were designed with 60 nm and 130 nm g
...